Brooksville is a city in western Florida and the county seat of Hernando County, Florida, United States. As of the 2010 census it had a population of 7,719, up from 7,264 at the 2000 census. Brooksville is home to historic buildings and residences, including the homes of former Florida Governor William Sherman Jennings and football player Jerome Brown. It is part of the Tampa-St. Petersburg-Clearwater, Florida Metropolitan Statistical Area.

Brooksville, established in 1856 by the merger of the towns of Melendez and Pierceville, took its name to honor and show support for Preston Brooks, a pro-slavery congressman from South Carolina who caned and seriously injured Massachusetts Senator and abolitionist Charles Sumner.